Shannon Miller, PhD
Assistant Professor
Department of Chemistry
Research Focus
Genome editing holds promise as a one-time cure for genetic disease, but issues with toxicity, types of DNA manipulations, and biomolecule delivery limit therapeutic application. The Miller Lab develops and applies technologies for the in vivo use of gene editing tools for the safe and effective study and treatment of genetic disease. We tackle problems spanning biology and chemistry, with specific emphasis on protein engineering, directed evolution, viral and non-viral delivery, and genome editing. Our research is currently focusing on three major areas: (1) the development of novel AAV vectors for improved in vivo use and industrial production, (2) the development of a novel method for large DNA manipulations, and (3) improving in vivo CRISPR therapeutics using fully chemically modified sgRNAs.
Select Publications
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Huang, Tony P.; Heins, Zachary J.; Miller, Shannon M.; Wong, Brandon G.; Balivada, P A.; Wang, Tina; Khalil, Ahmad S.; Liu, David R. High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. 2023, 41, 96-107.
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miller, shannon m.; liu, david r.; weiss, mitchell j.; tsai, shengdar q.; pruett-miller, shondra m.; kalfa, theodosia a.; tisdale, john f.; mccaffrey, anton p.; koblan, luke w.; wang, tina; zhao, kevin t.; richter, michelle; henderson, jordana m.; sharma, akshay; lechauve, christophe; thaman, elizabeth; podracky, christopher j.; jang, yoonjeong; everette, kelcee a.; mayberry, kalin; yao, yu; porter, shaina n.; tillman, heather; li, yichao; lazzarotto, cicera r.; mayuranathan, thiyagaraj; woodard, kaitly j.; yen, jonathan; newby, gregory a. Base editing of haematopoietic stem cells rescues sickle cell disease in mice. Nature 2021.
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miller, shannon m.; liu, david r.; wang, tina Phage-assisted continuous and non-continuous evolution. Nature Protocols 2020.
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miller, shannon m.; liu, david r.; rees, holly a.; newby, gregory a.; matuszek, zaneta; huang, tony p.; shen, max w.; arbab, mandana; randolph, peyton b.; wang, tina Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nature Biotechnology 2020.
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miller, shannon m.; liu, david r.; savage, david f.; fellmann, christof; oakes, benjamin l.; gaudelli, nicole m.; zhao, kevin t.; huang, tony p. Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nature Biotechnology 2019.
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miller, shannon m.; hennighausen, lothar; liu, chengyu; liu, david r.; smith, harold c.; willi, michaela; lee, hye k. Simultaneous targeting of linked loci in mouse embryos using base editing. Scientific Reports 2019.
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